Sample prepping gets better all the time – cleaner, faster, and cheaper. One of the reasons for better speed and cleaner sampling is the arrival of disposable polypropylene pipette tips, that you throw away after a single use. These really lower processing time, and they do away with any fear of cross-contamination.
One of the old bottlenecks in laboratory processing is solid-phase extraction. It’s entirely possible to use disposable tips for this too.
The general idea in disposable-tip extraction is that you’ve got a tip with sorbent in it, either powdered or dispersed between two disks, or ‘frits’, as they’re called, which allow bidirectional fluid flow but prevent contamination of the syringe. You can get reversed-phase sorbents, for non-polar to medium polar compounds, you can get strong cation exchanger sorbents, and weak anion exchanger sorbents, and so on.
One of our popular products here at Next Day is the Cryo-Safe™ freeze controller from Bel Art. It’s a well-engineered piece of equipment. It absolutely assures a drop in cell temperature of 1°C per minute, and a lot of labs want that, at high precision.
But where does that 1°C gradient rule come from? And is it right for every possible application?
It’s a fair question, and not every person in every lab is always clear about this. So here’s an answer, first in the form of a review of the general challenges in cryopreservation, and then a bit of discussion about cooling rates, and how they’re derived.
The premium workmanship of the robust die-cast
aluminum housing, coupled with the finest engineering materials and design,
provides excellent protection against mechanical and electrical interference.
This allows the balance to operate at the highest levels of precision, from the
initial weigh-in period through the final result. With features such as
self-calibration system and the intuitive user settable operation, the balance
will handle any task you set for it. For more information, review the
specifications of the individual balances.
The first centrifuge was built by Alexander Prandtl in 1875 as a mean of separating cream from milk. Since then, the laboratory centrifuge developed to be a principal tool in scientific and clinical labs.
The Centrifugation concept and applications:
When an object is put in rotation around an axis, an outward radial force is applied on it. This centripetal force is used to accelerate Sedimentation, a process where denser particles move faster to the bottom, or in a centrifuge, towards an outward direction. This principle is used for several different applications in the modern lab, with the purpose of separating different constitutes within a solution:
There are several types of filtration methods to choose from, which include gravity or simple, hot, and vacuum. Selecting the right method depends on the nature of the experiment. This guide will provide you with an easy to follow set of criteria that will help you choose the right filtration system for the job. The following chart is useful in getting you started.
The first step is to choose the right sized filter paper, which is the most vital step for vacuum filtration, because the filter must be smaller in diameter than the Hirsch or Buchner funnel. It needs to cover the holes and sit flat on the funnel bottom and not have any creases or folds. There are two ways to fold the filter paper, which are the fluted and conventional methods.
Membrane filtration utilizes pressure in order to force water or any other kind of carrier fluid through a porous or semi-permeable membrane. This process separates the particulate matter that's suspended from the soluble and fluid components.
Membrane filters are also known as membranes are microporous films which have specific ratings for their pore sizes. These are also known as microporous filters, screens or sieves, and they keep the microorganisms or particles which are bigger than the size of their pores through the process of surface capture. On the other hand, any particles smaller than the pore size of the membrane filter are typically kept by other types of mechanisms.
When choosing a filter to perform various applications or obtain certain objectives, there are several things one must consider. This guide will help you choose the right filter in just a few steps. Firstly, you match the application with the best pore size for the job. Secondly, you will select the best housing material and membrane for the application. Thirdly, you will select the right membrane area to help optimize the throughput and flow rate. And lastly, choose the filter design that best fits your application.
One of the most critical steps in successful cell isolation is the preparation of the single cell suspension. Cell loss may occur during isolation if the sample contains cell clumps during the cell separation process. Additionally, the clumps may interfere with the proper labeling of the cells that the process is meant to target. By following a specialized protocol for harvesting and preparing the single cell suspensions from primary or frozen tissue before the process of cell separation, these issues can be avoided.
Autoclaving is used to ensure equipment and supplies are properly sterilized by using pressurized steam at high temperatures to eliminate the present of any microorganisms that may be present. The process is used in various industries, including dentistry, medicine, microbiology, and veterinary science. Autoclaving is a reliable process that ensures equipment and supplies are decontaminated and sterilized. The process takes between thirty minutes to an hour and reaches a high temperature of around 250 degrees Fahrenheit.
When you work in a medical laboratory, you'll get exposed to different kinds of lab instruments and equipment. Therefore, anybody working in medical laboratories will be familiar with serological pipettes. These are laboratory instruments used for transferring liquid from one vessel to another. They have gradations along the sides. These are important for the measurement of the quantity of liquid that's dispensed or aspirated.
Cell culture plates are used in laboratories to provide optimum conditions for cell culture. A cell culture plate provides the right conditions for the growth of cell cultures. They are usually transparent to allow visual assaying, and the dishes can be either V-shaped, flat, or round at the bottom. They often have lids to protect the samples which might be placed in multiple wells for storage, experimentation, and screening.
While not all cell types require cell culture plating and can grow well in a liquid suspension, others called adherent cells need a surface onto which to latch. Most cells that have been sampled from solid tissues are adherent, so require a cell culture plate for growth and observation.
Spreaders, often known as cell spreaders, are tools used in the laboratory that allow for samples to be smoothly spread onto a petri-dish or plate. Chiefly used in the biological field with cell and bacterial samples, spreaders are made in three main shapes: the L-shape, the T-shape, and the triangular shape.
They are manufactured from various materials, depending on their function. These materials include glass, metal, or even plastic these days. Each of these materials has distinct advantages and disadvantages.
Glass, for example, can easily be sterilized for reuse time and time again. On the downside, though, glass is fairly easy to break. Broken glass, in turn, poses a potential danger to researchers in the laboratory. On the other hand, plastic does not need sterilization because these spreaders come ready-sterilized.
concern yourself when you work in laboratories, especially if you use solutions
and chemicals. Containers for these chemicals should be safe for handlers. This
is important to avoid any incidents that can cause injuries.
have been relying for decades on glass bottles because they are resistant to
most solutions and chemicals. However, they break easily. This property could
be a safety risk, especially if their contents are dangerous. The advent of
plastics has given us plastic bottles, which is a very safe alternative. These
cost much less than glass bottles. Their proper care and use can give you years
of dependable performance.
Glass out-performs plastic with its high chemical resistance against many substances, including acids, alkalis, organic solvents, saline solutions, and water. The only substances that can destroy glass are hydrofluoric acid, strong alkalis used at high temperatures, and concentrated phosphoric acid.
Additional advantages to using glass in the lab include its dimensional stability, even at high temperatures, and its transparency. Other advantages include the fact that many sizes of many pieces of laboratory equipment are available, and glass is easy to clean. It is suitable for reagent and chemical storage, and Pyrex, a type of glass, is resilient to heat.
Beakers are one of the most oft-used pieces of equipment in the laboratory. At the most basic level, beakers can hold samples of whatever you’re studying. As they come in different sizes, they can be used for a multitude of other functions, such as preserving a small chemical reaction.
Experiments that result in a liquid product utilize beakers to catch the liquid. Beakers are also used in experiments involving chromatography and are widely used in research, education, and industry.