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Autoradiography film has been used for many years to detect radioactive molecules. In more recent years, autoradiography film has detected specific nonradioactive molecules using probes that catalyze chemiluminescence.
Distribution of radioactivity in a direct autoradiograph can be quantitated accurately by scanning the film using a densitometer. The sensitivity can be greatly increased by converting the emitted energy to light. Usually this is done by using intensifying screens or organic scintillators.
Light in detection systems based on chemiluminescence possesses similar benefits of sensitivity, but induces constraints imposed by the non-linear response of film to light. In both cases, this can be solved by pre-exposing the film to a hypersensitizing light flash and then cooling the film to -70°C which gives a linear film response to light, increasing sensitivity for low signals and allowing accurate quantitation of the autoradiographic image.
100 sheets come with each package of any of three available items of autoradiography film which includes Blu-Lite UHC, Blu-Ray and BluBlot HS.
The Blu-Lite UHC is a high contrast, high sensitivity autoradiography film that is specially formulated for an optimized balance of high sensitivity and low background. This low background, high contrast formula produces a lighter blue film, making it ideal for all autoradiographic and electrophoretic applications. It is also coated on both sides for improved consistency and overall performance. It reliably produces sharp images which allow for maximum DNA visualization with unsurpassed clarity. Compatible with automatic processors and manual development methods, Blu-Lite compares directly with films costing 3-5 times as much and has a shelf life of two years from date of production.
BluBlot HS is a high quality, blue sensitive film that is ideal for a wide range of electrophoretic and autoradiography applications. It can be used with calcium tungstate or blue-emitting rare-earth screens. BluBlot HS exhibits excellent sensitivity and contrast characteristics and is coated on both sides for improved consistency and overall performance. It reliably produces sharp, bright images that are easily visualized and suitable for publication. Compatible with automatic processors and manual development methods, BluBlot HS compares directly with films costing up to three times as much. With a shelf life of 2 years from date of production, BluBlot HS is packaged in lined, light-safe dispenser boxes of 100 sheets per box.
Blu-Ray is an especially sensitive blue film, suitable for all general purpose autoradiography applications. With high contrast, a reduced background and double sided, it’s ideal for chemiluminescence.
