Cryogenic freezing is a process of cold preservation that is accomplished with very low temperatures. We’re talking a bone-chilling -238° F or -150° C. The storage method is derived from the science of cryogenics, which is about creating very cold environments and studying what the results are for samples that experience extremely low temperatures.
The term Tissue Culture may not evoke the most exciting of visions as a scientific pursuit, but the reality couldn't be more different. Think of scientists working in labs, coaxing life in Petri dishes from a few cells, and you begin to have a glimpse of what tissue culture is all about. When you are growing tissue or cells separately from the organism where they originated, that's Tissue Culture. The growth is usually promoted by using a growth medium, such as broth or agar. Tissue culture, a term invented by an early 20th century Midwestern pathologist, revolves specifically around the growth of animal and cell tissues. Plant tissue culture refers specifically to plants.
A Brief History
The science of biology has its origins in the early scientific work of two centuries ago. It began with a German zoologist named Wilhelm Roux, who removed part of a chicken embryo and kept it alive in saline for several days in 1885. Next, another zoologist grew nerve cells from a frog embryo cell set. By the 1990s, regenerative tissue replaced a small piece of a urethra and mammalian embryonic stem cell growth was developed. The same principles applied to plant tissue culture, where it was posited as early as 1902 that every plant cell had the capacity to develop a whole plant.