Enduro™ Modular Vertical PAGE System

Enduro™ Horizontal Gel Boxes

Labnet's Enduro Vertical PAGE System is modular - allowing PAGE, electroblotting and 2D gel electrophoresis to be carried out in the same tank, simply by changing the inserts. Users can purchase only the parts they need, or a complete combined system for a cost savings over buying all three systems separately. NDE2010-P Enduro PAGE System, includes PAGE insert, buffer tank with leads and colling pack $487       NDE2010-PB Enduro PAGE Electroblotting System, includes the blotting insert and PAGE system components $644       NDE2010-P2 Enduro PAGE 2D System, includes 2D insert and PAGE System components $674     NDE2010-BT Enduro Electroblotting System includes buffer tank and blotting insert $408     NDE2010-BM Electroblotting Module only for use with Enduro PAGE System $286     NDE2010-2M 2D Electrophoresis Module only for use with Enduro PAGE System $286     Additional spacers, combs and plates are available. Contact customer service at 866-650-0314 SPECIFICATIONS Enduro PAGE System Enduro PAGE Electroblotting System Enduro PAGE 2D System Gel Dimensions 10 x 10 cm NA 8 cm long Capacity 2 gels/run* 3 gels/transfer 10 tubes/run Maximum samples 20 per gel NA 10 (1/gel) Tank (WxDxH) 19 x 13 x 15 cm 19 x 13 x 15 cm 19 x 13 x 15 c, Buffer volume 250 mL 1200 mL 500 mL Included Items** 2 ea 10 x 10 notched plates 3 ea blotting cassettes 20 ea capillary tues 2 ea 10 x 10 plates w/spacers 6 ea fiber pads 20 ea tube supports 2 ea 1 mm comb, 12 wells Blotting module, PAGE System 10 ea blocking plugs PAGE Module, casting base IEF module PAGE System

Enduro HOrizontal Gel Boxes have been designed for ease of use, safety and durability.  A variety of sizes are available, from a mini gel system to a large maxi gel system that can accomodate as many as 450 samples in a single run. A wide range of accessories are available to customize your system, including multichannel compatible gel combs. Enduro 7.7 Ideal for quick runs and low to medium throughput of samples. NDE1007-7 $232   Gel: 7 x 7 cm Unit: 9 x 21 x 9 cm Buffer volume: 225 ml Max. samples: 32 Supplied combs: 2ea 8 tooth     Enduro 7.10 Small footprint, with longer gel for higher sample throughput or better resolution. NDE1007-10 $241 Gel: 7 x 10 cm Unit: 9 x 21 x 9 cm Buffer volume: 225 ml Max. samples: 64 Supplied combs: 3ea 8 tooth     Enduro 10.10 Ideal for quick checks of samples from PCR products. For medium throughput laboratories. NDE1010-10 $255 Gel: 10 x 10 cm Unit: 12.5 x 22 x 9 cm Buffer volume: 300 ml Max. samples: 100 Supplied combs: 2ea 10 tooth*     Enduro 10.15 Wide gel with low buffer requirements. NDE1015-10   $296 Gel: 10 x 15 cm Unit: 17.5 x 26.5 x 9 cm Buffer volume: 500 ml Max. samples: 140 Supplied combs: 1ea 16 tooth* 1 ea 20 tooth     Enduro 15.15 This gel offers both high throughput and excellent resolution of samples. NDE1015-15   $389 Gel: 15 x 15 cm Unit: 17.5 x 26.5 x 9 cm Buffer volume: 500 ml Max. samples: 210 Supplied combs: 1ea 16 tooth* 1 ea 20 tooth 1 ea 18 tooth*     Enduro 20.20 Excellent resolution, capacity for up to 450 samples. Ideal for rapid screening of large numbers of samples. NDE1020-20 $408 Gel: 20 x 20 cm Unit: 23 x 39.5 x 9 cm Buffer volume: 1,200 ml Max. samples: 450 Supplied combs: 2ea 40 tooth 2 ea 20 tooth*

Modular System The modularity of the Enduro System is based on three operational inserts and a common buffer tank.  All three inserts, PAGE, electroblotting and 2D electrophoresis, have their own electrode assembly that connects into the lid of the buffer tank. PAGE Insert Set up of gels is quick and easy with the PAGE insert. A set of plates are placed on either side of the insert and held into place by the pressure bar. An additional gel may be run on each side by stacking the plates.  The assembly is then placed into the casting stand for pouring the gel. After the gel is polymerized, the insert is removed from the casting stand and placed in the buffer tank. Electroblotting Insert The electroblotting insert has a capacity for 3 gels.  Setting up blots is easy- gels are sandwiched in the blotting cassettes with the transfer membranes.  The cassettes are then placed in the insert, which is in turn placed in the buffer tank. Coiled electrodes in the insert generate a high intensity current, allowing transfer to take place in about an hour. 2D Electrophoresis Insert This insert is used for running the first dimension of IEF gels. Up to ten 8cm gels are poured in disposable capillary tubes and placed in this insert. Gasketed ports allow for easy insertion and removal of tubes. Blocking plugs are included for cloing off ports that are not inuse. Accepts Most Precast Gels Most precast gels, including NuSep™, Bio-Rad™, Invitrogen™ and Cambrex™ can be used with the Enduro PAGE System.  The use of precast gels saves time by eliminating the need to pour gels and wait while they polymerize. Built for Safety and Endurance For leakproof performance and a long service life, the buffer tank is molded from high quality acrylic.  Pins on the top of the inserts ensure that the lid is placed onto the insert in the proper orientation.  These pins also aid in removal of the lid. Removing the lid disconnects power to the electrodes. Wide Range of Accessories A variety of combs and spacers of varying widths are available to customize your Enduro system. All are color coded and clearly marked for easy identification.  Both notched and plain plates can be purchased with spacers permanently bound for easy set up.  Additional fiber pads and replacement cassettes are also available as well as capillary tubes, blanking ports and tube supports. Cooling packs are also available to help reduce buffer temperature during higher voltage or longer runs. Enduro Packages Four Enduro packages are available.  The Enduro PAGE system includes the PAGE insert, casting stand, plates, combs and buffer tank. The Enduro PAGE Electroblotting System includes all of the components of the PAGE system plus the electroblotting insert, three cassettes and six fiber pads.  The tube gel insert and PAGE system components make up the Enduro 2D Electrophoresis System.  An electroblotting package is also available that includes the buffer tank, electroblotting insert and accessories.  All components are also available separately.

Quality Construction The Enduro gel tanks are molded for leakproof performance and a long service life.  The corrosion resistant platinum electrodes are located in cassettes to protect them during cleaning and provide for easy replacement.  The lids fit on the gel boxes in only one direction. A drip ring on the lid recycles condensed buffer and prevents it from dripping directly onto the gel. Ease of Use Casting gels for the Enduro system is very easy.  Simply snap the rubber gates onto the ends of the tray, place the comb in position and pour the gel - no taping is necessary. Slots on the sides of the gel trays hold the combs in place. The two piece combs are designed so that the teeth can be raised or lowered to vary the depth of the well within the gel.  The supplied combs are 1.5 mm thick. Combs of other thicknesses are available separately.  After the gel has hardened, the combs and gates are removed and the gel and tray placed in the gel box.  Loading strips may be placed on the bottom of the gel box to aid in well visualization. Gel trays are UV transparent.  The power leads for the Enduro system are attached to the gel box lid.  The connection with the electrodes is made only when the lid is properly in place. Removal of the lid removes easy and prevent sloshing of buffer.